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Registro Completo |
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Biblioteca(s): |
Embrapa Uva e Vinho. |
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Data corrente: |
17/12/2010 |
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Data da última atualização: |
31/10/2019 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SILVA, G. A. da; BERNARDI, T. L.; SCHAKER, P. D. C.; MELLO, L. M. R. de; VALENTE, P. |
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Afiliação: |
GILDO ALMEIDA DA SILVA, CNPUV; TAÍS LETÍCIA BERNARDI, UFRGS; PATRÍCIA DAYANE CARVALHO SCHAKER, UERGS; LOIVA MARIA RIBEIRO DE MELLO, CNPUV; PATRÍCIA VALENTE, UFRGS. |
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Título: |
Captan and Folpan inhibition of PCR amplification of 375 bp DNA fragment specific for the 18S region of Saccharomyces. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
In: INTERNATIONAL CONFERENCE ON CULTURE COLLECTIONS, 12., 2010, Florianópolis. Biological Resource Centrers: gateway to biodiversity and services for innovations in biotechnology: [abstracts]. [S.l]: WFCC, 2010. |
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Páginas: |
Não paginado. |
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Idioma: |
Inglês |
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Notas: |
Poster. |
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Conteúdo: |
The off-flavor metabolites produced by nutritionally fastidious yeasts can be economically disastrous to the wine producer since they render the wine unpalatable, decreasing the quality of the wine, and resulting in a lower sale price. These microorganisms are difficult to be detected by traditional culture media since these techniques involve complex enrichment. The polymerase chain reaction (PCR)is a well established molecular technique that uses tiny amounts of genetic material to be amplified, it does not depend on culture media for cell growth and so it can overcome several difficulties. This essentially enzymatic reaction can be employed to detect microorganisms in foods and beverages but it can also be susceptible to inhibitors. Molecular systematic investigation of members of the ascomycetous yeast genera or monitoring spoilage microorganisms in wine may not be an easy task due the presence of inhibitor compounds. Besides the presence of wine naturally occurring inhibitors, cultural practices, like fungicide treatments can also contribute to add external inhibiting factors if residues of these compounds are present in wine. The inhibition is likely to be dependent upon the residue levels. Captan and Folpan are widely employed in Brazilian vineyards to control fungal threats. The purpose of this work was to detect the action of these compounds upon Taq polymerase using a forward primer Fungf (5'-CTTAGTTGGTGGAGTGATTTG-3') and a reverse primer Fungr (5'-GGTACTAGCGACGGGCG-3') that amplify a 375 bp DNA fragment specific for the 18S region of fungi. The efficiency of PCR and yeast metabolism were evaluated with the strain Saccharomyces cerevisiaeEmbrapa 1vvt/97 (BRM-4687) in the presence of different concentrations of Captan (1200-recommended dose, 600, 300, 150, 75, and 37.5 ?g/mL) and Folpan (1350-recommended dose, 675, 337.5, 168.8, 84.4 and 42.2 ?g/mL). The amplification was observed only at 75, and 37.5 ?g/mL Captan. Negligible amplification was obtained at 84.4 ?g/mL Folpan and visible amplification was possible only at 42.2 ?g/mL Folpan. Captan and Folpan are, depending on the concentration, strong Taq polymerase inhibitors. The presence of both fungicides can make the hard work of monitoring process of spoilage microorganisms in wine even harder. These fungicides also showed to be strong inhibitors of the cell yeast metabolism.Key words:Fermentation, fungicides, PCR, Taq polymerase, yeast MenosThe off-flavor metabolites produced by nutritionally fastidious yeasts can be economically disastrous to the wine producer since they render the wine unpalatable, decreasing the quality of the wine, and resulting in a lower sale price. These microorganisms are difficult to be detected by traditional culture media since these techniques involve complex enrichment. The polymerase chain reaction (PCR)is a well established molecular technique that uses tiny amounts of genetic material to be amplified, it does not depend on culture media for cell growth and so it can overcome several difficulties. This essentially enzymatic reaction can be employed to detect microorganisms in foods and beverages but it can also be susceptible to inhibitors. Molecular systematic investigation of members of the ascomycetous yeast genera or monitoring spoilage microorganisms in wine may not be an easy task due the presence of inhibitor compounds. Besides the presence of wine naturally occurring inhibitors, cultural practices, like fungicide treatments can also contribute to add external inhibiting factors if residues of these compounds are present in wine. The inhibition is likely to be dependent upon the residue levels. Captan and Folpan are widely employed in Brazilian vineyards to control fungal threats. The purpose of this work was to detect the action of these compounds upon Taq polymerase using a forward primer Fungf (5'-CTTAGTTGGTGGAGTGATTTG-3') and a reverse primer Fungr (5'-GGTACTAGCGACGGGC... Mostrar Tudo |
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Palavras-Chave: |
Levedura; PCR; Polimerase. |
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Thesagro: |
Fermentação; Fungicida; Microbiologia; Vinho. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/195411/1/ab-gildo.pdf
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Marc: |
LEADER 03362nam a2200265 a 4500
001 1870250
005 2019-10-31
008 2010 bl uuuu u00u1 u #d
100 1 $aSILVA, G. A. da
245 $aCaptan and Folpan inhibition of PCR amplification of 375 bp DNA fragment specific for the 18S region of Saccharomyces.$h[electronic resource]
260 $aIn: INTERNATIONAL CONFERENCE ON CULTURE COLLECTIONS, 12., 2010, Florianópolis. Biological Resource Centrers: gateway to biodiversity and services for innovations in biotechnology: [abstracts]. [S.l]: WFCC$c2010
300 $aNão paginado.
500 $aPoster.
520 $aThe off-flavor metabolites produced by nutritionally fastidious yeasts can be economically disastrous to the wine producer since they render the wine unpalatable, decreasing the quality of the wine, and resulting in a lower sale price. These microorganisms are difficult to be detected by traditional culture media since these techniques involve complex enrichment. The polymerase chain reaction (PCR)is a well established molecular technique that uses tiny amounts of genetic material to be amplified, it does not depend on culture media for cell growth and so it can overcome several difficulties. This essentially enzymatic reaction can be employed to detect microorganisms in foods and beverages but it can also be susceptible to inhibitors. Molecular systematic investigation of members of the ascomycetous yeast genera or monitoring spoilage microorganisms in wine may not be an easy task due the presence of inhibitor compounds. Besides the presence of wine naturally occurring inhibitors, cultural practices, like fungicide treatments can also contribute to add external inhibiting factors if residues of these compounds are present in wine. The inhibition is likely to be dependent upon the residue levels. Captan and Folpan are widely employed in Brazilian vineyards to control fungal threats. The purpose of this work was to detect the action of these compounds upon Taq polymerase using a forward primer Fungf (5'-CTTAGTTGGTGGAGTGATTTG-3') and a reverse primer Fungr (5'-GGTACTAGCGACGGGCG-3') that amplify a 375 bp DNA fragment specific for the 18S region of fungi. The efficiency of PCR and yeast metabolism were evaluated with the strain Saccharomyces cerevisiaeEmbrapa 1vvt/97 (BRM-4687) in the presence of different concentrations of Captan (1200-recommended dose, 600, 300, 150, 75, and 37.5 ?g/mL) and Folpan (1350-recommended dose, 675, 337.5, 168.8, 84.4 and 42.2 ?g/mL). The amplification was observed only at 75, and 37.5 ?g/mL Captan. Negligible amplification was obtained at 84.4 ?g/mL Folpan and visible amplification was possible only at 42.2 ?g/mL Folpan. Captan and Folpan are, depending on the concentration, strong Taq polymerase inhibitors. The presence of both fungicides can make the hard work of monitoring process of spoilage microorganisms in wine even harder. These fungicides also showed to be strong inhibitors of the cell yeast metabolism.Key words:Fermentation, fungicides, PCR, Taq polymerase, yeast
650 $aFermentação
650 $aFungicida
650 $aMicrobiologia
650 $aVinho
653 $aLevedura
653 $aPCR
653 $aPolimerase
700 1 $aBERNARDI, T. L.
700 1 $aSCHAKER, P. D. C.
700 1 $aMELLO, L. M. R. de
700 1 $aVALENTE, P.
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Registro original: |
Embrapa Uva e Vinho (CNPUV) |
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| Registros recuperados : 16 | |
| 2. |  | BERNARDI, T. L.; SCHAKER, P. D. C.; TODESCHINI, A. P.; VALENTE, P.; SILVA, G. A. da. Ação do ácido gálico e da catequina na inibição da Reação em Cadeira da Polimerase. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA, 8.; ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 4., 2010, Bento Gonçalves. Resumos. Bento Gonçalves: Embrapa Uva e Vinho, 2010. p. 23. Resumo.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 5. | | SILVA, G. A. da; POLI, J. S.; POLETTO, C. M.; SCHAKER, P. D. C.; VALENTE, P. Production of functional killer protein in batch cultures upon a shift from aerobic to anaerobic conditions. Brazilian Archives of Biology and Technology, Curitiba, v. 54, n. 3, p. 601-612, maio/jun. 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Agroenergia; Embrapa Uva e Vinho. |
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| 6. | | NALIN, R.; AUGUSTINI, B. C.; VALENTE, P.; BERNARDI, T. L.; SILVA, G. A. da. Primer universal para leveduras não detecta a presença de Bacillus megaterium. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA, 9.; ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 5., 2011, Bento Gonçalves. Resumos... Bento Gonçalves: Embrapa Uva e Vinho, 2011. p. 19. Resumo.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 7. | | BERNARDI, T. L.; SCHAKER, P. D. C.; MENEGOTTO, M.; VALENTE, P.; SILVA, G. A. da. Inibição da reação em cadeia da polimerase por taninos do vinho. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA UVA E VINHO, 7.; ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 3., 2009, Bento Gonçalves. Resumos... Bento Gonçalves: Embrapa Uva e Vinho, 2009. p. 53.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 9. | | SILVA, G. A. da; BERNARDI, T. L.; SCHAKER, P. D. C.; MENEGOTTO, M.; VALENTE, P. Rapid yeast DNA extraction by boiling and freeze-thawing without using chemical reagents and DNA purification. Brazilian Archives of Biology and Technology, Curitiba, v. 55, n. 2, p. 319-327, mar./abr. 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 10. | | BERNARDI, T. L.; SCHAKER, P. D. C.; TODESCHINI, A. P.; VALENTE, P.; SILVA, G. A. da. Uso da enzima Tth DNA polimerase para amplificação direta de DNA de leveduras contido em vinho. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA, 8.; ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 4., 2010, Bento Gonçalves. Resumos. Bento Gonçalves: Embrapa Uva e Vinho, 2010. p. p. 38. Resumo.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 12. | | SILVA, G. A. da; BERNARDI, T. L.; MENEGOTTO, M.; SCHAKER, P. D. C.; BERLESI, M. S.; VALENTE, P. Inibição da reação em cadeia da polimerase por compostos presentes no vinho. In: CONGRESO LATINOAMERICANO DE VITICULTURA Y ENOLOGIA, 12., 2009, [Montevideo]. [Anais...] [Montevideo]: Asociación de Enólogos del Uruguay, 2009. Não paginado. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 14. | | SILVA, G. A. da; POLETTO, C. M.; POLI, J. S.; BALBINOTTE, J.; CARBONERA, A. de S.; SOUZA, E. de; LOCATELLI, C.; VALENTE, P. Comportamento de diferentes linhagens de Saccharomyces cerevisiae na presença da levedura Brattanomyces custersianus durante a fermentação tumultuosa da vinificação em tinto. In: CONGRESSO BRASILEIRO DE VITICULTURA E ENOLOGIA, 12., 2008, Bento Gonçalves. Anais... Bento Gonçalves: Embrapa Uva e Vinho, 2008. p. 157. Resumo.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 15. | | POLETTO, C. M.; SILVA, G. A. da; POLI, J. S.; BALBINOTTE, J.; CARBONERA, A.; BARBOSA, E. dos S.; LOCATELLI, C.; VALENTE, P. Inibição do metabolismo de diferentes linhagens de Saccharomyces cerevisiae induzida por Brettanomyces custersianus. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA UVA E VINHO, 6.; ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 2., 2008, Bento Gonçalves. Resumos... Bento Gonçalves: Embrapa Uva e Vinho, 2008. p. 49. Resumo.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| Registros recuperados : 16 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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